Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes

Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due its antioxidant properties. The present study investigated the antigenotoxic potential of enriched fraction Romina strawberry methanolic extract (ACY) against DNA damage human peripheral blood leukocytes, induced by hydrogen peroxide vitro. Five concentrations ACY were used all experiments (2.5, 5, 10, 15, 20 µg/ml). results alkaline comet assay showed no genotoxic effect ACY. After pre-exposure leukocytes ACY, and subsequent incubation with H2O2, a decreased number damaged cells was recorded tested concentrations, compared controls. In posttreatment, there concentration-dependent reduction, while statistically significant decrease achieved 15 µg/ml concentrations. indicate that is efficient oxidative it more potent as post-applicative than protective agent.